Rabbit anti-TRAF3 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TRAF3
CAS Number
9007-83-4
Synonyms
CAP1; CRAF1; TNF receptor-associated factor 3; CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1
Host
Rabbit
Reactivity
H, M, R, P, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF3. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TRAF3 expression in MCF7 (A), HeLa (B), Raji (C), NIH3T3 (D), mouse lung (E), rat colon (F), PC12 (G) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
7187
Gene ID (Mouse)
22031
SwissProt (Human)
Q13114
SwissProt (Mouse)
Q60803
Available Sizes
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