Rabbit anti-TRAF3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to TRAF3

CAS Number

9007-83-4

Synonyms

CAP1; CRAF1; TNF receptor-associated factor 3; CAP-1; CD40 receptor-associated factor 1; CRAF1; CD40-binding protein; CD40BP; LMP1-associated protein 1; LAP1

Host

Rabbit

Reactivity

H, M, R, P, S

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAF3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of TRAF3 expression in MCF7 (A), HeLa (B), Raji (C), NIH3T3 (D), mouse lung (E), rat colon (F), PC12 (G) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of TRAF3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .