Rabbit anti-STAT5A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to STAT5A
CAS Number
9007-83-4
Synonyms
STAT5; Signal transducer and activator of transcription 5A
Host
Rabbit
Reactivity
H, M, R, B, P, S
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human STAT5A. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of STAT5A expression in A431 (A), K562 (B), HeLa (C), NIH3T3 (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of STAT5A staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
6776
Gene ID (Mouse)
20850
Gene ID (Rat)
24918
SwissProt (Human)
P42229
SwissProt (Mouse)
P42230
SwissProt (Rat)
Q62771
Available Sizes
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