Rabbit anti-S6K1 (pT421) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to S6K1 (pT421)
CAS Number
9007-83-4
Synonyms
STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA
Host
Rabbit
Reactivity
H, M, R, B, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human S6K1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of S6K1 (pT421) expression in MDAMB231 insulin-treated (A), Jurkat (B), HeLa (C), NIH3T3 (D), PC12 (E) whole cell lysates.
IHC description
Immunohistochemical analysis of S6K1 (pT421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
6198
Gene ID (Mouse)
72508
Gene ID (Rat)
83840
SwissProt (Human)
P23443
SwissProt (Mouse)
Q8BSK8
SwissProt (Rat)
P67999
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