Rabbit anti-PCNA Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PCNA
CAS Number
9007-83-4
Synonyms
Proliferating cell nuclear antigen; PCNA; Cyclin
Host
Rabbit
Reactivity
H, M, R, B, C, D, Mk, P, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PCNA expression in HeLa (A), SP2/0 (B), H9C2 (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PCNA staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5111
Gene ID (Mouse)
18538
Gene ID (Rat)
25737
SwissProt (Human)
P12004
SwissProt (Mouse)
P17918
SwissProt (Rat)
P04961
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
14566091 4x 500 ng
C5orf25-set siRNA/shRNA/RNAi Lentivector (Human)
Sign In for Pricing
View Details 