Rabbit anti-GUCY1B3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GUCY1B3

CAS Number

9007-83-4

Synonyms

GUC1B3; GUCSB3; GUCY1B1; Guanylate cyclase soluble subunit beta-1; GCS-beta-1; Guanylate cyclase soluble subunit beta-3; GCS-beta-3; Soluble guanylate cyclase small subunit

Host

Rabbit

Reactivity

H, M, R, B, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GUCY1B3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GUCY1B3 expression in HEK293T (A), mouse kidney (B), rat kidney (C) whole cell lysates.

IHC description

Immunohistochemical analysis of GUCY1B3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.