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Rabbit anti-GAD1/2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GAD1/2

CAS Number

9007-83-4

Synonyms

GAD1; GAD; GAD67; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform; GAD2; GAD65; Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

Host

Rabbit

Reactivity

H, M, R, B, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GAD1/2. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GAD1/2 expression in SKNSH (A), SP2/0 (B), PC12 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

2571; 2572

Gene ID (Mouse)

14415; 14417

SwissProt (Human)

Q99259; Q05329

SwissProt (Mouse)

P48318; P48320

Available Sizes

Frequently Asked Questions

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