Rabbit anti-GAD1/2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GAD1/2
CAS Number
9007-83-4
Synonyms
GAD1; GAD; GAD67; Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform; GAD2; GAD65; Glutamate decarboxylase 2; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
Host
Rabbit
Reactivity
H, M, R, B, D, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GAD1/2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GAD1/2 expression in SKNSH (A), SP2/0 (B), PC12 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2571; 2572
Gene ID (Mouse)
14415; 14417
SwissProt (Human)
Q99259; Q05329
SwissProt (Mouse)
P48318; P48320
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