Rabbit anti-Collagen 4 alpha 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Collagen 4 alpha 1
CAS Number
9007-83-4
Synonyms
Collagen alpha-1 (IV) chain
Host
Rabbit
Reactivity
H, M, B, C, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 4 alpha 1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Collagen 4 alpha 1 expression in HEK293T (A), mouse endothelial (B) whole cell lysates.
IHC description
Immunohistochemical analysis of Collagen 4 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1282
Gene ID (Mouse)
12826
SwissProt (Human)
P02462
SwissProt (Mouse)
P02463
Available Sizes
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