Rabbit anti-Laminin gamma 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Laminin gamma 1
CAS Number
9007-83-4
Synonyms
LAMB2; Laminin subunit gamma-1; Laminin B2 chain; Laminin-1 subunit gamma; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-2 subunit gamma; Laminin-3 subunit gamma; Laminin-4 subunit gamma; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit gamma; Laminin-9 subunit gamma; S-laminin subunit gamma; S-LAM gamma
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant protein of human Laminin gamma 1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Laminin gamma 1 expression in LO2 (A), mouse kidney (B), rat kidney (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Laminin gamma 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Desmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
3915
SwissProt (Human)
P11047
SwissProt (Mouse)
P02468
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC400525-100 1x 100 µL
CD63 (MX-49.129.5), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400031-100 1x 100 µL
Mucin 5AC (MUC5AC) (45M1), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680204-500 1x 500 µL
Complement C4d (C4D204), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC880531-500 1x 500 µL
VEGF-R2 / CD309 / Flk-1 / KDR3 (DC101), CF488A conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC941014-500 1x 500 µL
ACTH (Adrenocorticotrophic Hormone) (2F6), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details