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Rabbit anti-Peroxiredoxin 5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Peroxiredoxin 5

CAS Number

9007-83-4

Synonyms

ACR1; Peroxiredoxin-5, mitochondrial; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Peroxiredoxin 5

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Peroxiredoxin 5 expression in Hela (A), HepG2 (B), mouse liver (C), mouse heart (D), rat brain (E) whole cell lysates.

IHC description

Immunohistochemical analysis of Peroxiredoxin 5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Peroxiredoxin 5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

25824

Gene ID (Mouse)

54683

Gene ID (Rat)

113898

SwissProt (Human)

P30044

SwissProt (Mouse)

P99029

SwissProt (Rat)

Q9R063

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