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Rabbit anti-Surfactant Protein C Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Surfactant Protein C

CAS Number

9007-83-4

Synonyms

SFTP2; Pulmonary surfactant-associated protein C; SP-C; Pulmonary surfactant-associated proteolipid SPL (Val) ; SP5

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human Surfactant Protein C

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Surfactant Protein C expression in Jurkat (A), Raji (B) whole cell lysates.

IHC description

Immunohistochemical analysis of Surfactant Protein C staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Surfactant Protein C staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6440

Gene ID (Rat)

50683

SwissProt (Human)

P11686

SwissProt (Mouse)

P21841

SwissProt (Rat)

P11685

Available Sizes

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