Rabbit anti-HSD17B2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HSD17B2
CAS Number
9007-83-4
Synonyms
EDH17B2; Estradiol 17-beta-dehydrogenase 2; 17-beta-hydroxysteroid dehydrogenase type 2; 17-beta-HSD 2; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E2DH; Microsomal 17-beta-hydroxysteroid dehydrogenase; Testosterone 17-beta-dehydrogenase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human HSD17B2
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HSD17B2 expression in SW480 (A), mouse liver (B), mouse kidney (C) whole cell lysates.
IHC description
Immunohistochemical analysis of HSD17B2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of HSD17B2 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
3294
Gene ID (Mouse)
15486
Gene ID (Rat)
79243
SwissProt (Human)
P37059
SwissProt (Mouse)
P51658
SwissProt (Rat)
Q62730
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
