Rabbit anti-Cullin 4A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Cullin 4A
CAS Number
9007-83-4
Synonyms
Cullin-4A; CUL-4A
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide of human Cullin 4A
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Cullin 4A expression in Hela (A), HepG2 (B), mouse brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Cullin 4A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Cullin 4A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8451
Gene ID (Mouse)
99375
SwissProt (Human)
Q13619
SwissProt (Mouse)
Q3TCH7
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