Rabbit anti-Beclin-1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Beclin-1
CAS Number
9007-83-4
Synonyms
GT197; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Beclin-1
Clonality
Polyclonal
Applications
WB, IHC, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Beclin-1 expression in A431 (A), MCF7 (B), Raji (C), mouse kidney (D), rat brain (E) whole cell lysates.
IHC description
Immunohistochemical analysis of Beclin-1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
8678
Gene ID (Mouse)
56208
Gene ID (Rat)
114558
SwissProt (Human)
Q14457
SwissProt (Mouse)
O88597
SwissProt (Rat)
Q91XJ1
Available Sizes
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