Rabbit anti-APTX Antibody
Product Specifications
Background
Rabbit polyclonal antibody to APTX
CAS Number
9007-83-4
Synonyms
AXA1; Aprataxin; Forkhead-associated domain histidine triad-like protein; FHA-HIT
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human APTX
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of APTX expression in A549 (A), K562 (B), Raji (C) whole cell lysates.
IHC description
Immunohistochemical analysis of APTX staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of APTX staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
54840
Gene ID (Mouse)
66408
Gene ID (Rat)
259271
SwissProt (Human)
Q7Z2E3
SwissProt (Mouse)
Q7TQC5
SwissProt (Rat)
Q8K4H4
Available Sizes
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