Rabbit anti-BAF53B Antibody
Product Specifications
Background
Rabbit polyclonal antibody to BAF53B
CAS Number
9007-83-4
Synonyms
ACTL6; BAF53B; Actin-like protein 6B; 53 kDa BRG1-associated factor B; Actin-related protein Baf53b; ArpNalpha; BRG1-associated factor 53B; BAF53B
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human BAF53B
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BAF53B expression in HL60 (A), Hela (B), HepG2 (C), mouse brain (D) whole cell lysates.
IHC description
Immunohistochemical analysis of BAF53B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of BAF53B staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
51412
Gene ID (Mouse)
83766
Gene ID (Rat)
288563
SwissProt (Human)
O94805
SwissProt (Mouse)
Q99MR0
SwissProt (Rat)
P86173
Available Sizes
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