What is the catalog number for Rabbit anti-PIST Antibody?

The catalog number for Rabbit anti-PIST Antibody is DL96477A.

What sizes are available for Rabbit anti-PIST Antibody?

Rabbit anti-PIST Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-PIST Antibody come in?

Rabbit anti-PIST Antibody is available in 2 different pack sizes.

Is Rabbit anti-PIST Antibody in stock?

Rabbit anti-PIST Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-PIST Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PIST

CAS Number

9007-83-4

Synonyms

CAL; FIG; Golgi-associated PDZ and coiled-coil motif-containing protein; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10; PIST

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PIST

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PIST expression in HepG2 (A), Hela (B), mouse kidney (C), mouse liver (D), rat brain (E) whole cell lysates.

IHC description

Immunohistochemical analysis of PIST staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of PIST staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.