Rabbit anti-RbAp46 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RbAp46
CAS Number
9007-83-4
Synonyms
RBAP46; Histone-binding protein RBBP7; Histone acetyltransferase type B subunit 2; Nucleosome-remodeling factor subunit RBAP46; Retinoblastoma-binding protein 7; RBBP-7; Retinoblastoma-binding protein p46
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human RbAp46
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of RbAp46 expression in HT29 (A), MCF7 (B), mouse heart (C), mouse ovary (D), rat spleen (E), rat lung (F) whole cell lysates.
IHC description
Immunohistochemical analysis of RbAp46 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of RbAp46 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5931
Gene ID (Mouse)
245688
Gene ID (Rat)
83712
SwissProt (Human)
Q16576
SwissProt (Mouse)
Q60973
SwissProt (Rat)
Q71UF4
Available Sizes
Curated Selection
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