Rabbit anti-FIR Antibody
Product Specifications
Background
Rabbit polyclonal antibody to FIR
CAS Number
9007-83-4
Synonyms
FIR; ROBPI; SIAHBP1; Poly (U) -binding-splicing factor PUF60; 60 kDa poly (U) -binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human FIR
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of FIR expression in A549 (A), mouse brain (B), mouse spleen (C), rat lung (D), rat liver (E) whole cell lysates.
IHC description
Immunohistochemical analysis of FIR staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of FIR staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
22827
Gene ID (Mouse)
67959
SwissProt (Human)
Q9UHX1
SwissProt (Mouse)
Q3UEB3
SwissProt (Rat)
Q9WV25
Available Sizes
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