Rabbit anti-HuC Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HuC
CAS Number
9007-83-4
Synonyms
HUC; PLE21; ELAV-like protein 3; Hu-antigen C; HuC; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic limbic encephalitis antigen 21
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide of human HuC
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/20 - 1/50)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HuC expression in mouse brain (A), rat brain (B) whole cell lysates.
IHC description
Immunohistochemical analysis of HuC staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1995
Gene ID (Mouse)
15571
SwissProt (Human)
Q14576
SwissProt (Mouse)
Q60900
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