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Rabbit anti-HuC Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HuC

CAS Number

9007-83-4

Synonyms

HUC; PLE21; ELAV-like protein 3; Hu-antigen C; HuC; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic limbic encephalitis antigen 21

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide of human HuC

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/20 - 1/50)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HuC expression in mouse brain (A), rat brain (B) whole cell lysates.

IHC description

Immunohistochemical analysis of HuC staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1995

Gene ID (Mouse)

15571

SwissProt (Human)

Q14576

SwissProt (Mouse)

Q60900

Available Sizes

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