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Rabbit anti-FIH-1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to FIH-1

CAS Number

9007-83-4

Synonyms

FIH1; Hypoxia-inducible factor 1-alpha inhibitor; Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human FIH-1

Clonality

Polyclonal

Applications

WB, IHC, IF/IC, IP, ChIP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/50 - 1/200), ChIP (1/20 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of FIH-1 expression in MCF7 (A), K562 (B), mouse heart (C) whole cell lysates.

IHC description

Immunohistochemical analysis of FIH-1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of FIH-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

55662

Gene ID (Mouse)

319594

SwissProt (Human)

Q9NWT6

SwissProt (Mouse)

Q8BLR9

Available Sizes

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