Rabbit anti-VAP-B/C Antibody
Product Specifications
Background
Rabbit polyclonal antibody to VAP-B/C
CAS Number
9007-83-4
Synonyms
Vesicle-associated membrane protein-associated protein B/C; VAMP-B/VAMP-C; VAMP-associated protein B/C; VAP-B/VAP-C
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human VAP-B/C
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of VAP-B/C expression in SW480 (A), HepG2 (B), Raji (C), mouse brain (D) whole cell lysates.
IHC description
Immunohistochemical analysis of VAP-B/C staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of VAP-B/C staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
9217
Gene ID (Rat)
60431
SwissProt (Human)
O95292
SwissProt (Mouse)
Q9QY76
SwissProt (Rat)
Q9Z269
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TA500629BM 100 µL
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