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Rabbit anti-ABAT Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ABAT

CAS Number

9007-83-4

Synonyms

GABAT; 4-aminobutyrate aminotransferase, mitochondrial; (S) -3-amino-2-methylpropionate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human ABAT

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ABAT expression in SW480 (A), mouse kidney (B), mouse brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of ABAT staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of ABAT staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

18

Gene ID (Mouse)

268860

Gene ID (Rat)

81632

SwissProt (Human)

P80404

SwissProt (Mouse)

P61922

SwissProt (Rat)

P50554

Available Sizes

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