What is the catalog number for Rabbit anti-eIF4A1 Antibody?

The catalog number for Rabbit anti-eIF4A1 Antibody is DL96043A.

What sizes are available for Rabbit anti-eIF4A1 Antibody?

Rabbit anti-eIF4A1 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-eIF4A1 Antibody come in?

Rabbit anti-eIF4A1 Antibody is available in 2 different pack sizes.

Is Rabbit anti-eIF4A1 Antibody in stock?

Rabbit anti-eIF4A1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-eIF4A1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to eIF4A1

CAS Number

9007-83-4

Synonyms

DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human eIF4A1

Clonality

Polyclonal

Applications

WB, IF/IC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of eIF4A1 expression in Hela (A), Jurkat (B), NIH3T3 (C), PC12 (D), mouse liver (E) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of eIF4A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.