Rabbit anti-eIF4A1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to eIF4A1
CAS Number
9007-83-4
Synonyms
DDX2A; EIF4A; Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human eIF4A1
Clonality
Polyclonal
Applications
WB, IF/IC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of eIF4A1 expression in Hela (A), Jurkat (B), NIH3T3 (C), PC12 (D), mouse liver (E) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of eIF4A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
1973
Gene ID (Mouse)
13681
SwissProt (Human)
P60842
SwissProt (Mouse)
P60843
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