Rabbit anti-p54nrb Antibody

Product Specifications

Background

Rabbit polyclonal antibody to p54nrb

CAS Number

9007-83-4

Synonyms

NRB54; Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex, 52 kDa subunit; NMT55; p54 (nrb) ; p54nrb

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human p54nrb

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of p54nrb expression in Hela (A), SW620 (B), mouse brain (C), mouse kidney (D) whole cell lysates.

IHC description

Immunohistochemical analysis of p54nrb staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.