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Rabbit anti-PSMA4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PSMA4

CAS Number

9007-83-4

Synonyms

HC9; PSC9; Proteasome subunit alpha type-4; Macropain subunit C9; Multicatalytic endopeptidase complex subunit C9; Proteasome component C9; Proteasome subunit L

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PSMA4

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PSMA4 expression in HepG2 (A), Jurkat (B), mouse liver (C), COS7 (D), PC12 (E) whole cell lysates.

IHC description

Immunohistochemical analysis of PSMA4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of PSMA4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

5685

Gene ID (Mouse)

26441

Gene ID (Rat)

29671

SwissProt (Human)

P25789

SwissProt (Mouse)

Q9R1P0

SwissProt (Rat)

P21670

Available Sizes

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