What is the catalog number for Rabbit anti-PPP2R2A Antibody?

The catalog number for Rabbit anti-PPP2R2A Antibody is DL95991A.

What sizes are available for Rabbit anti-PPP2R2A Antibody?

Rabbit anti-PPP2R2A Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-PPP2R2A Antibody come in?

Rabbit anti-PPP2R2A Antibody is available in 2 different pack sizes.

Is Rabbit anti-PPP2R2A Antibody in stock?

Rabbit anti-PPP2R2A Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-PPP2R2A Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PPP2R2A

CAS Number

9007-83-4

Synonyms

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A subunit B isoform alpha

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PPP2R2A

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PPP2R2A expression in Hela (A), Jurkat (B), mouse brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of PPP2R2A staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of PPP2R2A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.