What is the catalog number for Rabbit anti-LC3B Antibody?

The catalog number for Rabbit anti-LC3B Antibody is DL95753A.

What sizes are available for Rabbit anti-LC3B Antibody?

Rabbit anti-LC3B Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-LC3B Antibody come in?

Rabbit anti-LC3B Antibody is available in 2 different pack sizes.

Is Rabbit anti-LC3B Antibody in stock?

Rabbit anti-LC3B Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-LC3B Antibody

Product Specifications

Background

Rabbit polyclonal antibody to LC3B

CAS Number

9007-83-4

Synonyms

MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant protein corresponding to human LC3B.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/200 - 1/500), IF/IC (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of LC3B expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of LC3B staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of LC3B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .