Rabbit anti-Cyclin E1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Cyclin E1
CAS Number
9007-83-4
Synonyms
CCNE; G1/S-specific cyclin-E1
Host
Rabbit
Reactivity
H, M, R, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin E1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Cyclin E1 expression in HepG2 (A), MCF7 (B), K562 (C), mouse lung (D) whole cell lysates.
IHC description
Immunohistochemical analysis of Cyclin E1 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of Cyclin E1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
898
Gene ID (Mouse)
12447
SwissProt (Human)
P24864
SwissProt (Mouse)
Q61457
SwissProt (Rat)
P39949
Available Sizes
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