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Rabbit anti-ATF2 (pS62) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ATF2 (pS62)

CAS Number

9007-83-4

Synonyms

CREB2; CREBP1; Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1

Host

Rabbit

Reactivity

H, M, R, C

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATF2. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ATF2 (pS62) expression in MCF7 UV-treated (A), A549 (B), mouse brain (C), rat liver (D) whole cell lysates.

IHC description

Immunohistochemical analysis of ATF2 (pS62) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1386

Gene ID (Mouse)

102641666; 11909

Gene ID (Rat)

81647

SwissProt (Human)

P15336

SwissProt (Mouse)

P16951

SwissProt (Rat)

Q00969

Available Sizes

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