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Rabbit anti-Aquaporin 4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Aquaporin 4

CAS Number

9007-83-4

Synonyms

Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Aquaporin 4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Aquaporin 4 expression in HeLa (A), Jurkat (B), PC12 (C), SP2/0 (D), HepG2 (E), U87MG (F), mouse brain (G) whole cell lysates.

IHC description

Immunohistochemical analysis of Aquaporin 4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

361

Gene ID (Mouse)

11829

Gene ID (Rat)

25293

SwissProt (Human)

P55087

SwissProt (Mouse)

P55088

SwissProt (Rat)

P47863

Available Sizes

Frequently Asked Questions

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