Rabbit anti-GNE Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GNE
CAS Number
9007-83-4
Synonyms
GLCNE; Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human GNE
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GNE expression in A549 (A), Jurkat (B), mouse liver (C), mouse lung (D), rat liver (E) whole cell lysates.
IHC description
Immunohistochemical analysis of GNE staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
10020
Gene ID (Mouse)
50798
Gene ID (Rat)
114711
SwissProt (Human)
Q9Y223
SwissProt (Mouse)
Q91WG8
SwissProt (Rat)
O35826
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