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Rabbit anti-WDR51A Antibody

Product Specifications

Background

Rabbit polyclonal antibody to WDR51A

CAS Number

9007-83-4

Synonyms

WDR51A; POC1 centriolar protein homolog A; Pix2; Proteome of centriole protein 1A; WD repeat-containing protein 51A

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human WDR51A

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of WDR51A expression in HL60 (A), SKOV3 (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of WDR51A staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

25886

Gene ID (Mouse)

70235

SwissProt (Human)

Q8NBT0

SwissProt (Mouse)

Q8JZX3

Available Sizes

Frequently Asked Questions

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