Rabbit anti-SMC1A Antibody
Product Specifications
Background
Rabbit polyclonal antibody to SMC1A
CAS Number
9007-83-4
Synonyms
DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human SMC1A
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of SMC1A expression in Hela (A), SKOV3 (B), mouse liver (C), rat liver (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of SMC1A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
8243
Gene ID (Mouse)
24061
Gene ID (Rat)
63996
SwissProt (Human)
Q14683
SwissProt (Mouse)
Q9CU62
SwissProt (Rat)
Q9Z1M9
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