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Rabbit anti-PEG1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PEG1

CAS Number

9007-83-4

Synonyms

PEG1; Mesoderm-specific transcript homolog protein; Paternally-expressed gene 1 protein

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human PEG1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PEG1 expression in MCF7 (A), HT29 (B), mouse testis (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PEG1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

4232

Gene ID (Mouse)

17294

Gene ID (Rat)

58827

SwissProt (Human)

Q5EB52

SwissProt (Mouse)

Q07646

SwissProt (Rat)

Q6P5P5

Available Sizes

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