Rabbit anti-15-LO Antibody
Product Specifications
Background
Rabbit polyclonal antibody to 15-LO
CAS Number
9007-83-4
Synonyms
LOG15; Arachidonate 15-lipoxygenase; 15-LOX; 15-LOX-1; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type; 12-LOX; Arachidonate omega-6 lipoxygenase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human 15-LO
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of 15-LO expression in COS7 (A), mouse heart (B) whole cell lysates.
IHC description
Immunohistochemical analysis of 15-LO staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
246
Gene ID (Mouse)
11687
Gene ID (Rat)
81639
SwissProt (Human)
P16050
SwissProt (Mouse)
P39654
SwissProt (Rat)
Q02759
Available Sizes
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