Rabbit anti-TAT Antibody
Product Specifications
Background
Rabbit polyclonal antibody to TAT
CAS Number
9007-83-4
Synonyms
Tyrosine aminotransferase; TAT; L-tyrosine:2-oxoglutarate aminotransferase
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human TAT
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of TAT expression in HepG2 (A), mouse heart (B), rat liver (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of TAT staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
6898
Gene ID (Mouse)
234724
Gene ID (Rat)
24813
SwissProt (Human)
P17735
SwissProt (Mouse)
Q8QZR1
SwissProt (Rat)
P04694
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