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Rabbit anti-ALAS-E Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ALAS-E

CAS Number

9007-83-4

Synonyms

ALASE; ASB; 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human ALAS-E

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ALAS-E expression in HEK293T (A), mouse lung (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of ALAS-E staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

212

Gene ID (Mouse)

11656

Gene ID (Rat)

25748

SwissProt (Human)

P22557

SwissProt (Mouse)

P08680

SwissProt (Rat)

Q63147

Available Sizes

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