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Rabbit anti-AREB6 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to AREB6

CAS Number

9007-83-4

Synonyms

AREB6; TCF8; Zinc finger E-box-binding homeobox 1; NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human AREB6

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of AREB6 expression in SKOV3 (A), Hela (B), NIH3T3 (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of AREB6 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

6935

Gene ID (Mouse)

21417

SwissProt (Human)

P37275

SwissProt (Mouse)

Q64318

SwissProt (Rat)

Q62947

Available Sizes

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