Rabbit anti-Plastin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Plastin
CAS Number
9007-83-4
Synonyms
Plastin-3; T-plastin
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Plastin
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Plastin expression in MCF7 (A), A431 (B), HepG2 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Plastin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5358
Gene ID (Mouse)
102866
SwissProt (Human)
P13797
SwissProt (Mouse)
Q99K51
SwissProt (Rat)
Q63598
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