Rabbit anti-RAP Antibody

Product Specifications

Background

Rabbit polyclonal antibody to RAP

CAS Number

9007-83-4

Synonyms

A2MRAP; Alpha-2-macroglobulin receptor-associated protein; Alpha-2-MRAP; Low density lipoprotein receptor-related protein-associated protein 1; RAP

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide of human RAP

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of RAP expression in K562 (A), HepG2 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of RAP staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.