Rabbit anti-PP1 alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PP1 alpha
CAS Number
9007-83-4
Synonyms
PPP1A; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; PP-1A
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human PP1 alpha
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PP1 alpha expression in MCF7 (A), A549 (B), NIH3T3 (C), mouse brain (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of PP1 alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
5499
Gene ID (Mouse)
19045
Gene ID (Rat)
24668
SwissProt (Human)
P62136
SwissProt (Mouse)
P62137
SwissProt (Rat)
P62138
Available Sizes
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