Rabbit anti-HCK Antibody
Product Specifications
Background
Rabbit polyclonal antibody to HCK
CAS Number
9007-83-4
Synonyms
Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human HCK
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of HCK expression in HL60 (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of HCK staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
3055
Gene ID (Mouse)
15162
Gene ID (Rat)
25734
SwissProt (Human)
P08631
SwissProt (Mouse)
P08103
SwissProt (Rat)
P50545
Available Sizes
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