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Rabbit anti-ANP Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ANP

CAS Number

9007-83-4

Synonyms

ANP; PND; Natriuretic peptides A; CDD-ANF; Prepronatriodilatin

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human ANP

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ANP expression in mouse heart (A) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of ANP staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

4878

Gene ID (Mouse)

230899

Gene ID (Rat)

24602

SwissProt (Human)

P01160

SwissProt (Mouse)

P05125

SwissProt (Rat)

P01161

Available Sizes

Frequently Asked Questions

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