Rabbit anti-ANP Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ANP
CAS Number
9007-83-4
Synonyms
ANP; PND; Natriuretic peptides A; CDD-ANF; Prepronatriodilatin
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human ANP
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ANP expression in mouse heart (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of ANP staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4878
Gene ID (Mouse)
230899
Gene ID (Rat)
24602
SwissProt (Human)
P01160
SwissProt (Mouse)
P05125
SwissProt (Rat)
P01161
Available Sizes
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