Rabbit anti-Survivin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Survivin
CAS Number
9007-83-4
Synonyms
API4; IAP4; Baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor 4; Apoptosis inhibitor survivin
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human Survivin
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Survivin expression in K562 (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Survivin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
332
Gene ID (Mouse)
11799
Gene ID (Rat)
64041
SwissProt (Human)
O15392
SwissProt (Mouse)
O70201
SwissProt (Rat)
Q9JHY7
Available Sizes
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