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Rabbit anti-IBA1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to IBA1

CAS Number

9007-83-4

Synonyms

G1; IBA1; Allograft inflammatory factor 1; AIF-1; Ionized calcium-binding adapter molecule 1; Protein G1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human IBA1

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of IBA1 expression in mouse spleen (A) whole cell lysates.

IHC description

Immunohistochemical analysis of IBA1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

199

Gene ID (Mouse)

11629

Gene ID (Rat)

29427

SwissProt (Human)

P55008

SwissProt (Mouse)

O70200

SwissProt (Rat)

P55009

Available Sizes

Frequently Asked Questions

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