Rabbit anti-ATG5 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ATG5
CAS Number
9007-83-4
Synonyms
APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein
Host
Rabbit
Reactivity
H, M, R
Immunogen
Recombinant full length protein of human ATG5
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of ATG5 expression in HepG2 (A), NIH3T3 (B), mouse spleen (C), rat kidney (D) whole cell lysates.
IHC description
Immunohistochemical analysis of ATG5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
9474
Gene ID (Mouse)
11793
SwissProt (Human)
Q9H1Y0
SwissProt (Mouse)
Q99J83
SwissProt (Rat)
Q3MQ06
Available Sizes
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